Determination of Antimutagenic Effects of Some Foods and Drinks in vitro Binding Capacities of Some Dietary Fibers to Mutagens

Abstract

İn this study, mutagenic and antimutagenic activities o f various foods and drinks and in vitro binding capacities of certain dietary fiber and dietary fiber coııstitııents are studied. Raw, boiled juice and l e a f and dried seeds of Urtica sp., Stachys anımla, sage, rosehip, grape nıolasses and tarhana were not found mutagenic in Salmonella typhimıırium TA 100. Amoııg the foods tested, raw Urtica sp. (46.32%) and rosehip (44.03%) showed the highest antimutagenic effect, followed by boiled leaf of Urtica sp. (41.25%), boiled juice o f Urtica sp. (40.07%), sage (39.53), dried seeds o f Urtica sp. (37.22%), Stachys annııla (36.42%), grape molasses (33.03%) and tarhana (28.60%) respectively. The antimutagenic activities o f Urtica sp. and rosehip were f o und to be sigııificantly higher thaıı the antimutagenic activity of tarhana (p< 0.01). Direct acting mııtageıı sodiıım azid were not sorbed by dietary fiber (potato fib e r , glucomannan) and dietary fiber constituents (pectic acid, cellulose). Hovvever indirect acting mutagen 2- amino-3-methyl-3H-imidazo (4,5-f) quinoline was sorbed by them in variable ratios at 0°C, pH 4.5 and 37°C, PH 7.0. 2 -amino-3-methyl-3H-imidazo (4,5-f) cjuinoline (10) was not released from the dietary fibers and constituents in distilled water. 2.0-6.5 nıg IQ/g dietary fiber at 0°C, pH 4.5 and 2.2-3.8 mg IQ/g dietary fiber at 37°C, pH 7.0 were sorbed by dietary fibers and constituents. Amoııg the dietary fibers tested, potato fiber showed the highest binding capacity follo\ved by pectic acid, cellulose and glucomannan at 0°C, pH 4.5 condition.